![]() ![]() Estimate the signal and concentration detection limits and the lower limit of quantitation for EDTA. Low concentrations of EDTA near the detection limit gave the following dimensionless instrument readings: 175,104,164,193,131,189,155,133,151, and 176. To measure accuracy and precision of the method, blood was fortified with EDTA to known levels.Īccuracy = 100 × mean value found - known value known value Precision = 100 × standard deviation mean = coeffcient of variationįor each of the three spike levels in the table, find the precision and accuracy of the quality control samples. This trial motivated the development of a new method to measure EDTA in blood. It should be noted that, in this study, where we present the analysis of a SR-based pre-emphasis method of neural spikes for the first time, we use a monostable well potential. Even though the amount of EDTA found in the crime-scene blood was orders of magnitude below ~ 4. Second, spike detection performance degrades for some overlapping spikes. At the time of the trial, procedures to measure EDTA in blood were not well established. 5 mM after the vial is filled with blood. Blood is normally collected in a vial containing the metal-binding compound EDTA (as an anticoagulant) at a concentration of ~ 4. The defense argued that police "planted" the defendant's blood from a sample collected later. The prosecutor argued that blood was left by the defendant during the crime. In a murder trial in the 1990 s, the defendant's blood was found at the crime scene. What is the percent relative uncertainty in the quotient / ? Why do we avoid 3-point calibration curves? Spikes focus on gathering information and finding answers to a questions, rather than producing a shippable product. From the standard uncertainty in (b), compute the 95 % confidence interval for the quotient /. 1 Minutes Definition of a Spike: Spike is the name for a timeboxed user story or Task that is created in order to research a question or resolve a problem. Find the value of Student's for confidence and 1 degree of freedom. For n = 3 data points, there is n - 2 = 1 degree of freedom, because 2 degrees of freedom are lost in computing the slope and intercept. (c) Here is why we try not to use 3-point calibration curves. Find the standard uncertainty for the peak area ratio. (b) Find the quotient role="math" localid="1663559638520" / for an unknown whose peak area ratio ( C 10 H 8 / C 10 D 8 ) is 0.652. What is the theoretical value of the intercept? Is the observed value of the intercept within experimental uncertainty of the theoretical value? Find the least-squares slope and intercept and their standard uncertainties. (a) Using a spreadsheet such asFigure 4-15, prepare a graph of Equation 5-12 showing peak area ratio ( C 10 H 8 / C 10 D 8 ) versus concentration ratio role="math" localid="1663559632352" ( / C 10 D 8 ). The two compounds emerge from the column at almost identical times and are measured by a mass spectrometer. Internal standard graph - Data are shown below for chromatographic analysis of naphthalene ( C 10 H 8 ), using deuterated naphthalene ( C 10 D 8, in which D is the isotope 2 H ) as an internal standard. ![]()
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